Supporting online material for: Transcriptional Regulatory Networks in Saccharomyces cerevisiae
نویسندگان
چکیده
Epitope Tagging of Strains Regulators were tagged by inserting multiple copies of the Myc epitope coding sequence into the normal chomosomal loci of these genes. The plasmid pWZV88 (1) was used as a template to generate PCR products containing the Myc epitope coding sequence and a selectable marker (TRP) flanked by homologous regions designed to recombine at the 3’ end of the targeted transcriptional regulator. PCR products were transformed into the W303 strain Z1256. Clones were selected for growth on TRPplates. Insertion of the epitope coding sequence was confirmed by PCR and expression of the epitope-tagged protein was confirmed by Western blotting using an anti-Myc antibody.
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